Cloning of the <Emphasis Type="Italic">cnr</Emphasis> operon into a strain of <Emphasis Type="Italic">Bacillaceae</Emphasis> bacterium for the development of a suitable biosorbent |
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| Authors: | Elvis Fosso-Kankeu Antoine F Mulaba-Bafubiandi Lizelle A Piater Matsobane G Tlou |
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| Institution: | 1.School of Chemical and Minerals Engineering, Faculty of Engineering,North-West University,Potchefstroom,South Africa;2.Minerals Processing and Technology Research Center, Department of Extraction Metallurgy, School of Mining, Metallurgy and Chemical Engineering, Faculty of Engineering and the Built Environment,University of Johannesburg,Doornfontein,South Africa;3.Department of Biochemistry, Faculty of Science,University of Johannesburg,Auckland Park,South Africa |
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| Abstract: | In this study, a potential microbial biosorbent was engineered to improve its capacity to remediate heavy metal contaminated water resources. A Bacillaceae bacterium isolated from a mining area was transformed with a plasmid carrying the (pECD312)-based cnr operon that encodes nickel and cobalt resistance. The bioadsorption ability of the transformed strain was evaluated for removal of nickel from metallurgical water relative to the wildtype strain. Results showed that transformation improved the adsorption capacity of the bacterium by 37 % at nickel concentrations equivalent to 150 mg/L. Furthermore it was possible to apply prediction modelling to study the bioadsorption behaviour of the transformed strain. As such, this work may be extended to the design of a nickel bioremediation plant utilising the newly developed Bacillaceae bacterium as a biosorbent. |
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