Cell size as a tool to predict the production of recombinant protein by the insect-cell baculovirus expression system |
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| Authors: | Laura A Palomares Juan Carlos Pedroza Octavio T Ramírez |
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| Institution: | (1) Departamento de Bioingeniería, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P. 510-3 Cuernavaca Morelos, México;(2) Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, México |
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| Abstract: | The increase of Sf9 cell diameter after infection with a recombinant baculovirus encoding VP8 protein of rotavirus can be used to predict culture productivity. A direct proportional correlation between the increase in cell size and VP8 concentration was obtained when manipulating selected medium components. Only yeast extract increased (38%) VP8 concentration, while fetal bovine serum increased (55%) the maximum cell concentration. An inexpensive and simplified culture media can thus be designed without detriment to protein yields. |
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| Keywords: | baculovirus culture monitoring insect cell size metabolism recombinant protein |
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