Penehyclidine hydrochloride attenuates LPS-induced iNOS production by inhibiting p38 MAPK activation in endothelial cells |
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Authors: | Jia Zhan Zong-ze Zhang Chang Chen Kai Chen Yan-lin Wang |
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Institution: | (1) Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, People’s Republic of China; |
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Abstract: | The aim of this study was to investigate the inhibitory effect of penehyclidine hydrochloride (PHC) on lipopolysaccharide
(LPS)-induced nitric oxide (NO) and inducible nitric oxide synthase (iNOS) production in human endothelial cell. Cultured
endothelial cells were pretreated with PHC, followed by LPS treatment. NO activity were determined. iNOS expression and p38
mitogen-activated protein kinase (p38 MAPK) protein expression were measured by Western blot analysis. LPS treatment significantly
induced p38 MAPK activation, iNOS expression, and NO production, which could be attenuated by 2 μg/ml PHC pretreatment. Furthermore,
our study showed LPS-induced NO production and iNOS expression were suppressed by p38 MAPK inhibitor SB203580 pretreatment.
We concluded that PHC attenuates NO production and iNOS expression by suppressing the activation of p38 MAPK pathway, thereby
implicating a mechanism by which PHC may exert its protective effects against LPS-induced endothelial cell injury. |
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