The influence of reaction temperature on biomineralization of ferrihydrite cores in human H-ferritin |
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Authors: | Lanxiang Tian Changqian Cao Yongxin Pan |
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Institution: | (1) Paleomagnetism and Geochronology Laboratory, Key Laboratory of the Earth’s Deep Interior, Institute of Geology and Geophysics, Chinese Academy of Sciences, 100029 Beijing, People’s Republic of China;(2) Franco-Chinese Biomineralization and Nano-structures Laboratory, Chinese Academy of Sciences, 100029 Beijing, People’s Republic of China |
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Abstract: | Ferritin is not only important for iron storage and detoxification in living organisms, but a multifunctional size-constrained
nanoplatform for biomimetic nanoparticles. In order to tailor the biomimetic nanoparticles for future applications, it is
essential to investigate the effects of external factors such as temperature on the particle size and structure of reconstituted
cores in ferritin. In this study, we systematically investigated the mineral composition, crystallinity, and particle size
of human H-ferritin (HuHF) reconstituted at four different temperatures (25, 30, 37, and 42°C) by integrated magnetic and
transmission electron microscopy analyses. Our results showed that the particle size of reconstituted ferrihydrite cores (~5 nm)
in HuHF was temperature-independent. However, the significant changes of the induced magnetization at 5 T field (M5T) and remanent magnetization (Mr) at 5 K clearly showed that the crystallinity of reconstituted cores increased with increasing temperature, indicating that
the reaction temperature deeply affects the structural order of reconstituted ferrihydrite cores rather than the particle
size, and the reconstituted cores become more ordered at higher reaction temperatures. Our findings provide useful insights
into biomineralization of ferritin under in vivo fever condition as well as in biomimetic synthesis of nanomaterials using
ferritin. Furthermore, the rock magnetic methods should be very useful approaches for characterizing finite ferritin nanoparticles. |
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