Sulfanilic acid‐modified chitosan mini‐spheres and their application for lysozyme purification from egg white |
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| Authors: | Daniela B. Hirsch María F. Baieli Nicolás Urtasun Juan M. Lázaro‐ Martínez Romina J. Glisoni María V. Miranda Osvaldo Cascone Federico J. Wolman |
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| Affiliation: | 1. Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología. Junín 956, 1113 Buenos Aires, Argentina;2. CONICET‐Universidad de Buenos Aires, Instituto de Nanobiotecnología (NANOBIOTEC), Junín 956, 1113 Buenos Aires, Argentina;3. Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Química Orgánica, Junín 956 (C1113AAD), Buenos Aires, Argentina;4. CONICET‐Universidad de Buenos Aires, Instituto de Química y Fisicoquímica Biológicas (IQUIFIB), Junín 956 (C1113AAD), Buenos Aires, Argentina;5. Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Tecnología Farmacéutica II. Junín 956, 1113 Buenos Aires, Argentina |
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| Abstract: | A cation exchange matrix with zwitterionic and multimodal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographic matrix was physico‐chemically characterized by ss‐NMR and ζ potential, and its chromatographic performance was evaluated for lysozyme purification from diluted egg white. The maximum adsorption capacity, calculated according to Langmuir adsorption isotherm, was 50.07 ± 1.47 mg g?1 while the dissociation constant was 0.074 ± 0.012 mg mL?1. The process for lysozyme purification from egg white was optimized, with 81.9% yield and a purity degree of 86.5%, according to RP‐HPLC analysis. This work shows novel possible applications of chitosan based materials. The simple synthesis reactions combined with the simple mode of use of the chitosan matrix represents a novel method to purify proteins from raw starting materials. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:387–396, 2018 |
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| Keywords: | multimodal ion exchange chromatography chitosan chromatographic matrix sulfanilic acid egg white lysozyme |
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