Optimization of a synthetic siRNA delivery for the treatment of rhabdomyosarcoma |
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| Authors: | Ewa Lesko Katarzyna Miekus Agnieszka Grabowska Aleksandra Gładys Marcin Majka |
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| Affiliation: | (1) Department of Transplantation, Polish-American Institute of Pediatrics, Jagiellonian University School of Medicine, 30-663 Krakow, Poland |
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| Abstract: | Silencing of a target-genes by small interfering RNA (siRNA) has emerged as a powerful new tool not only for basic research but also with potential therapeutic benefits. This paper demonstrates that optimal delivery strategy is crucial for effective target-gene silencing. Using lipofection, under defined conditions, we were able to markedly down-regulate expression of the selected genes involved in rhabdomyosarcoma metastasis: MET, CXCR4, LIFR and PAX3-FKHR. |
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| Keywords: | siRNA Rhabdomyosarcoma Lipofectamine 2000 MET receptor CXCR4 LIFR PAX3-FKHR |
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