Substance P and enkephalin in transected axons of medulla and spinal cord |
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Authors: | V M Pickel R Miller J Chan K K Sumal |
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Institution: | 1. Department of Pharmacology and Physiological Sciences, University of Chicago, Chicago, IL, U.S.A.;2. Laboratory of Neurobiology, Department of Neurology, Cornell University Medical College, 1300 York Avenue, New York, NY 10021, U.S.A. |
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Abstract: | The accumulation of transported materials in cut axons is demonstrated by the light and electron microscopic immunocytochemical localization of substance P and enkephalin in the caudal medulla and cervical spinal cord of adult rat. Two days following unilateral knife-cuts in the caudal medulla or spinal (C2-C3) levels, substance P and enkephalin-like immunoreactivity (SPLI and ELI) are detected in lesioned axons located rostral and caudal to the transection. Rostrally, SPLI and ELI are detected in the lateral reticular region and ventrolateral fasciculus corresponding to the location of previously identified bulbospinal pathways. Caudally, previously unidentified, propriospinal pathways showing SPLI are detected in the dorsal columns and in the dorsolateral fasciculus. In contrast, ELI is found caudal to the transection only in the reticular region of the medulla. For both peptides, immunoreactivity is present throughout axons containing numerous large, dense core, and small clear vesicles. These results support the concept of both particulate and soluble modes of transport for substance P and enkephalin within axons of the central nervous system. |
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Keywords: | immunocytochemistry neuropeptides transport lesion brain stem pathways 10 dorsal motor nucleus of the vagus 12 hypoglossal nucleus COM commissural nucleus of solitary tracts CC central canal CU cuneate nucleus and fasciculus DCS dorsal corticospinal tract DSC dorsal spinocerebellar tract GR gracilis nucleus and fasciculus LFU lateral funiculus spinal cord LRT lateral reticular nucleus MDD reticular nucleus of medulla dorsal MDV reticular nucleus of medulla ventral MLX medial lemniscus decussation PYR pyramidal decussation ROB raphe obscurus nucleus SP5 spinal tract of trigeminal nerve SP5C nucleus of the spinal tract of the trigeminal nerve caudal part SOL solitary tract VfU ventral funiculus spinal cord The layers of Rexed in the spinal cord are indicated by the numerals 1–9 |
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