精氨加压素片段(4-8)对鼠脑PKC和PKA活性的影响

精氨加压素的 C端片段 ,AVP( 4 - 8) ,具有增强记忆的功能 ,它在大鼠脑内引发一系列的生理生化反应 .PKC经常是 G蛋白偶联受体信号传导途径中的介导激酶 ,在 AVP( 4 - 8)信号转导支路中亦不例外 .放射性配基结合实验表明 ,在海马及皮层突触膜上存在 AVP( 4 - 8)的特异性结合位点 .AVP( 4 - 8)可以刺激大鼠脑内 PKC酶活的升高 ,并可以被 AVP( 4 - 8)的受体拮抗剂 ZDC( C) PR阻断 .在同样条件下 ,AVP( 4 - 8)对 PKA酶活无显著性影响 .

Effect of Arginine-Vasopressin(4-8)on PKC and PKA Activities in Rat Brain

AVP(4 8),one of the endogenous metabolites of arginine|vasopressin(AVP)in brain,can facilitate the acquisition and maintain of learning and memory.AVP(4 8) can induce a series of biochemical reactions in rat brain.PKC is an important mediator of signal transduction.The specific binding of AVP(4 8) on rat hippocampus was determined by radioligand binding assay.The activity of PKC in rat brain was significantly enhanced by AVP(4 8),and the enhancement of PKC was suppressed by ZDC(C)PR,an antagonist of AVP(4 8).Under the same conditions,the activity of PKA was not changed.