首页 | 本学科首页   官方微博 | 高级检索  
   检索      


The chondrocyte-intrinsic circadian clock is disrupted in human osteoarthritis
Authors:Sarah J B Snelling  Alana Forster  Supratim Mukherjee  Andrew J Price
Institution:1. Botnar Research Centre, University of Oxford, Oxford, United Kingdom;2. Department of Orthopaedics, MidCentral District Health Board, Palmerston North, New Zealand
Abstract:Peripheral clocks are essential for driving cell differentiation. In osteoarthritis, loss of the normal differentiated chondrocyte (cartilage cell) phenotype is causative of disease. We investigated whether clock gene expression differed in osteoarthritic compared to “healthy” chondrocytes and used RNAi to determine whether the differences observed could affect chondrocyte phenotype. Following serum shock, PER2 expression was significantly higher, whereas BMAL1 expression was significantly lower, in osteoarthritic chondrocytes. Knockdown of BMAL1 in “healthy” chondrocytes was associated with higher cell proliferation and MMP13 expression, features characteristic of the osteoarthritic chondrocyte phenotype. Chondrocyte-intrinsic clock disruption may be a critical early step in osteoarthritis development.
Keywords:Cartilage  cell phenotype  cell proliferation  peripheral circadian clocks  peripheral clocks in disease  matrix metalloproteinases
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号