Active Ca<sup>2+</sup> transport by vesicles reconstituted from triton X-100-solubilized pigeon erythrocyte membrane期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Active Ca²⁺ transport by vesicles reconstituted from triton X-100-solubilized pigeon erythrocyte membrane

Authors:	Wang K. Yeung Gary Weisman George A. Vidaver

Affiliation:	Department of Chemistry, University of Nebraska at Lincoln, Lincoln, NE 68588 U.S.A.

Abstract:	Pigeon erythrocyte membrane was solubilized partially, but relatively unselectively by Triton X-100. Vesicles were reconstituted from mixtures of Triton-solubilized membrane and lipid (phosphatidylcholine plus phosphatidylethanolamine plus cholesterol) by addition of bovine high-density lipoprotein. This efficiently removed the Triton X-100. Sodium dodecyl sulfate-polyacrylamide gel electropherograms of reconstituted vesicles showed band patterns resembling those of the original membrane. The reconstituted vesicles showed ATP-dependent active accumulation of ⁴⁵Ca²⁺. ATP-dependent ⁴⁵Ca²⁺ uptake by the reconstituted vesicles resembled the corresponding activity of the original membrane vesicles; in both preparations the Ca²⁺ uptake rate depended on the square of the Ca²⁺ concentration and had similar $[Ca^{2+}]_{12}$ values, 0.16 μM and 0.18 μM, respectively.

Keywords:	Membrane reconstruction Triton X-100 solubilization (Pigeon erythrocyte membrane) SDS sodium dodecyl sulfate HDL EGTA To whom reprint requests should be sent.
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