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Binding of unmodified low-density lipoproteins to human fibroblasts. An investigation by immunoelectron microscopy |
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| Authors: | B.J. Vermeer J.F. Koster J.J. Emeis F.C. Reman W.C. De Bruijn |
| Affiliation: | 1. Dept. of Dermatology, University Hospital, Rijnsburgerweg 10, Leiden The Netherlands;2. Dept. of Biochemistry I, Erasmus University, Rotterdam The Netherlands;3. Gaubius Institute, Health Research Organization TNO, Leiden The Netherlands;4. Lab. for Electron Microscopy, University of Leiden, Leiden The Netherlands |
| Abstract: | The binding of unmodified low density lipoproteins to the plasma membrane of fibroblasts was studied at the ultrastructural level. The bound low density lipoprotein was visualized by an indirect immunoperoxidase technique, with the use of an antiserum against apoprotein B. Immunoreactive regions representing bound apoprotein B were found on the plasma membrane, in indented regions with a diameter of 0.15–0.30 μm and a fuzzy coat on the cytoplasmic side. Fibroblasts from a patient homozygous for hyperlipoproteinaemia type IIa showed no immunoreactive material in the indented regions. The specific 125I-labelled low density lipoprotein binding to these homozygous fibroblasts was 7% compared to control fibroblasts. |
| Keywords: | Immunoperoxidase Ultrastructure LDL binding Immunoelectron microscopy (Fibroblast) LDL human low density lipoprotein (density range 1.019–1.063 g/ml) prepared by ultracentrifugation [10] |
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