Constraint on the substrate cytochrome binding reaction in bovine adrenocortical microsomes at physiological temperature |
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Authors: | Shakunthala Narasimhulu |
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Affiliation: | Harrison Department for Surgical Research, University of Pennsylvania, Philadelphia, PA 19174 U.S.A. |
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Abstract: | The addition of cholate to the microsomes at 37.5°C resulted in a striking decrease in the apparent substrate dissociation constant () and its temperature dependency. The microsomal membranes depleted of 80% of the lipids preserved the temperature dependency of the and exhibited breaks in the Van't Hoff plot at the characteristic temperature of the lipids phase transition. The results indicate that the cytochrome is considerably restrained from expressing its maximum substrate binding potential at physiological temperature. In addition, the results indicate that the majority of the lipids apparently do not play a significant role in imposing constraint on the substratecytochrome binding reaction and in the temperature dependency of the . |
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Keywords: | Membrane constraint Temperature dependence DPH 1,6-diphenyl-1,3,5-hexatriene ANS 1-anilinonaphthalene-6-sulfonate Hepes |
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