Relationship of growth temperature and thermotropic lipid phase changes in cytoplasmic and outer membranes from Escherichia coli K12 |
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Authors: | Andrew S Janoff A Haug Estelle J McGroarty |
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Institution: | 1. Department of Biophysics, Michigan State University, East Lansing, MI 48824 U.S.A.;2. MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824 U.S.A. |
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Abstract: | Purified cytoplasmic and outer membranes isolated from cells of wild type Escherichia coli grown at 12, 20, 37 and 43°C were labelled with the fatty acid spin probe 5-doxyl stearate. Electron spin resonance spectroscopy revealed broad thermotropic phase changes. The inherent viscosity of both membranes was found to increase as a function of elevated growth temperature. The lipid order to disorder transition in the outer membrane but not the cytoplasmic membrane was dramatically affected by the temperature of growth. As a result, the cytoplasmic membrane presumably existed in a gel + liquid crystalline state during cellular growth at 12 and 20°C, but in a liquid crystalline state when cells were grown at 37 and 43°C. In contrast, the outer membrane apparently existed in a gel + liquid crystalline state at all incubation temperatures. Data presented here indicate that the temperature range over which the cell can maintain the outer membrane phospholipids in a mixed (presumedly gel + liquid crystalline) state correlates with the temperature range over which growth occurs. |
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Keywords: | Temperature adaptation Phase transition Growth limit SDS sodium dodecyl sulfate 5-DS 5-doxyl stearate |
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