Induction of a chloride conductance in gastric vesicles by limited trypsin or chymotrypsin digestion or ageing |
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Authors: | E Rabon I Kajdos G Sachs |
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Institution: | Laboratory of Membrane Biology, University of Alabama Medical Center, Birmingham, AL 35294 U.S.A. |
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Abstract: | Transport activity of the hog gastric (H+ + K+)-ATPase system was measured either as the formation of a proton gradient using the dye probe acridine orange or as the formation of a proton diffusion potential using the cyanine dye 3,3′-diethyloxdicarbocyanine iodide in the presence of the protonophore tetrachlorosalicylanilide. The development of these gradients has been compared in K+ media in the presence of either Cl? or SO42? as the anionic species. This comparison of proton diffusion potential formation to proton gradient formation has been used to demonstrate that a Cl? conductance in this vesicular system results from limited enzymic digestion with either trypsin or α-chymotrypsin and from the ageing process itself. The possible significance of this finding is discussed. |
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Keywords: | Ageing Protease digestion Carbocyanine dye DOCC 3 3′-diethyloxodicarbocyanine iodide TCS tetrachlorosalicylanilide |
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