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Lipid reorganization in biological membranes. A study by fourier transform infrared difference spectroscopy
Authors:Hector L Casal  Ian CP Smith  David G Cameron  Henry H Mantsch
Institution:Division of Biological Sciences and Division of Chemistry, National Research Council of Canada, Ottawa, K1A 0R6 Canada
Abstract:The first application of infrared difference spectroscopy to the study of a natural biological membrane is described. Perdeuterated palmitic acid was incorporated biosynthetically into the lipids of the plasma membrane of Acholeplasma laidlawii and the temperature-induced structural rearrangement of the endogenous lipids monitored via their C2H vibrational modes. Changes in infrared parameters were studied between 0 and 50°C and contrasted with those occurring in the model membrane system of 1,2-diperdeuteropalmitoyl-sn-glycero-3-phosphocholine. The phase transition of the biomembrane occurs over a 20°C range with the temperature of the maximum rate of change of absorbance coinciding with that of the sharp phase transition of the model membrane.
Keywords:Lipid reorganization  Fourier transform  Phase transition  (Infrared difference)  1
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