Calcium-induced fusion of proteoliposomes and protein-free liposomes Effect of their phosphatidylethanolamine content on the structure of fused vesicles |
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Authors: | Alexander E. Gad Rachel Broza Gera D. Eytan |
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Affiliation: | Department of Biology, Technion, Israel Institute of Technology, Haifa Israel |
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Abstract: | The acidic phospholipid cardiolipin was shown to be very efficient in promoting calcium-induced fusion of proteoliposomes. The degree of fusion was dependent on the phosphatidylethanolamine content of the vesicles. Addition of CaCl2 to proteoliposomes containing phosphatidylcholine and cardiolipin but without phosphatidylethanolamine did not induce fusion. Fusion of cytochrome oxidase vesicles, containing less than 50 mol% phosphatidylethanolamine resulted in monolamellar vesicles with a diameter of about 200 nm. The vesicles could be induced to fuse further by establishing an osmotic pressure across their membranes. When proteoliposomes containing more than 50 mol% phosphatidylethanolamine were fused, large vesicles with a diameter exceeding 1 μm were formed. They appeared in the electron microscope as a mixture of multilamellar and monolamellar vesicles. Fusion of corresponding liposomes resulted in formation of even larger structures appearing as dense multilamellar bodies and paracrystalline honeycomb-like lattices. |
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Keywords: | Proteoliposome Liposome Phosphatidylethanolamine Cardiolipin Hepes FCCP |
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