17beta-estradiol affects proliferation and apoptosis of rat prostatic smooth muscle cells by modulating cell cycle transition and related proteins |
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Authors: | Luo Yi Waladali Wafi Li Shiwen Zheng Xinmin Hu Liquan Zheng Hang Hu Wanli Chen Chan |
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Affiliation: | Research Center of Urology and Andrology, Zhongnan Hospital, Wuhan University, 169# Donghu Road, Wuhan, Hubei 430071, PR China. |
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Abstract: | Abundant evidence indicates that estrogens have an important role in the pathology of benign prostatic hyperplasia (BPH). To investigate the effect of 17beta-estradiol (E2) on the proliferation and apoptosis of prostatic smooth muscle cells (PSMCs), rat PSMCs were obtained and exposed to gradient concentrations (0.1-100 nmol/l) of E2 over varying amounts of time. The progression of cell cycle, cellular apoptosis, cyclin D1, Bcl-2 and Bax proteins were detected. The data show that the effect of E2 on rat PSMCs is bilateral: it promotes cell proliferation by enhancing the expression of cyclin D1, which accelerates G1 to S phase transition; on the other hand, it induces apoptosis of the cells by up-regulating the expression of Bax. We thus suggest that an increase in estrogen may exert a launching effect in the pathology of BPH. |
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Keywords: | Estradiol Prostate Smooth muscle cell Proliferation Apoptosis Cell cycle |
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