Analysis of non-covalent aggregation of synthetic hPTH (1-34) by size-exclusion chromatography and the importance of suppression of non-specific interactions for a precise quantitation |
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Authors: | Kamberi Marika Chung Paul DeVas Richard Li Lily Li Zengji Ma Xiaoyan Sharon Fields Steven Riley Christopher M |
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Institution: | Department of Analytical Sciences, ALZA Corporation, Mountain View 94039-7210, 1501 California Avenue, Palo Alto, CA 94304, USA. mkamberi@alzus.jnj.com |
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Abstract: | There are few methods available for the rapid and precise quantitation of non-covalent aggregation. Size-exclusion chromatography (SEC), a traditional approach, used to measure the non-covalent aggregation can easily disrupt the weak forces holding an aggregate together. Under the conditions described in this paper the disaggregation of non-covalent aggregate of the synthetic human parathyroid hormone hPTH (1-34) due to hydrophobic/electrostatic interactions with the size-exclusion chromatography column packing was completely suppressed. This report details the effectiveness of adding salts and organic solvents in the mobile phase to overcome non-specific interactions that disrupt the aggregate during the SEC process and may aid in the understanding precise quantitation of non-covalent aggregation. |
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Keywords: | SEC PTH Non-covalent aggregation |
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