Globin synthesis in friend-erythroleukemia mouse cells in protein- and lipid-free medium : Effects of dimethyl-sulfoxide, iron and erythropoietin |
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| Authors: | N Kluge G Gaedicke G Steinheider S Dube W Ostertag |
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| Institution: | 1. Max-Planck-Institut für Experimentelle Medizin, Arbeitsgruppe Dube, 34 Göttingen, BRD;2. Molekularbiologisch-hämatologische Arbeitsgruppe an der Universitäts Kinderklinik, 2 Hamburg-Eppendorf, BRD;3. Max-Planck-Institut für Experimentelle Medizin, Abteilung Molekulare Biologie, BRD |
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| Abstract: | A cell clone of erythroleukemic mouse cells transformed by the spleen focus forming virus (SFFV) was adapted to growth in serum-free medium. The cells show induced erythroid differentiation if dimethylsulfoxide (DMSO) and iron are added to the serum-free medium. No serum constituents or macromolecules are required for induced differentiation. Serum enhances the capacity of the cells to differentiate. Erythropoietin is ineffective in promoting erythroid differentiation or an increased rate of cell division. Transferrin is not necessary for transport of iron into these cells. |
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