Control of Azospirillum brasilense NifA activity by PII: effect of replacing Tyr residues of the NifA N-terminal domain on NifA activity |
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| Authors: | Florence Arsènea PAlexandre Kaminskia Claudine Elmericha |
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| Institution: | Unité de Physiologie Cellulaire, Centre National de la Recherche Scientifique URA D1300, Département des Biotechnologies, Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris, France. |
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| Abstract: | It was previously reported that the N-terminal domain of Azospirillum brasilense NifA was a negative regulator of the NifA activity and that the P(II) protein prevented this inhibition under nitrogen fixing conditions. Here, we show that a mutation of a single Tyr residue at position 18 of the N-terminal domain of NifA led to an active NifA protein that did not require P(II) for activation under nitrogen fixation conditions. |
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| Keywords: | Nitrogen fixation NifA activity glnB Azospirillum brasilense |
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