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Genetic diversity of Darnel (Lolium temulentum L.) in Malo, Ethiopia depends on traditional farming systems
Authors:Takayuki Senda  Tohru Tominaga
Institution:(1) Graduate School of Agriculture, Kyoto Prefectural University, Ktaina-Yazuma, Seika, Soraku, 619-0244 Kyoto, Japan;(2) Graduate School of Agriculture, Kyoto Prefectural University, Seika, Kyoto, Japan
Abstract:Genetic Diversity of Darnel (Lolium temulentum L.) in Malo, Ethiopia Depends on Traditional Farming Systems. Economic Botany 58(4):568–577, 2004. Darnel (Lolium temulentum L.) is a mimic weed associated with wheat and barley cultivation. Mimic weeds have evolved along with cultivated crops. Human impact on the genetic diversity of agricultural weeds was elucidated using darnel as a model. Three strains in each field in Gaitza village, Malo region, Ethiopia were randomly sampled, and the genetic composition of 120 samples was analyzed using RAPD analysis. Genetic diversity and distances were calculated using Nei’s analysis, and cluster analysis was performed. These results indicated the occurrence of seed migration among the fields and a high genetic diversity of darnel in this region. Crop seed exchange and contamination of crop seeds with darnel seeds lead to the unintended artificial gene flow of darnel. Because darnel seeds and seedlings resemble their associated crop seeds and seedlings, traditional methods of crop cultivation have the unintended consequence of conserving the genetic diversity of darnel.
Keywords:Associated weed  darnel  genetic diversity            Lolium temulentum            RAPD  traditional farming
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