Prion protein gene polymorphisms in four goat breeds of Pakistan |
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Authors: | M E Babar M Abdullah A Nadeem A U Haq |
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Institution: | (1) Department of Livestock Production, University of Veterinary and Animal Sciences, Lahore, Pakistan;(2) Faculty of Animal Husbandry, University of Agriculture, Faisalabad, Pakistan |
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Abstract: | Four different goat breeds (Pak-Angora, Dera Din Panah, Naachi and Teddy) of Pakistan were selected to investigate polymorphism
in the prion protein gene (PrP gene) responsible for scrapie disease resistance in goats. Initially, genotyping of 187 animals
of these four breeds by restriction fragment length polymorphism (RFLP) was done to see the genotype for codon 136 and 154.
All the animals were monomorphic with a genotype of AARR except one animal of Teddy breed having the genotype of AARH. Sequencing
of PrP gene of twenty animals representing these four goat breeds revealed two genotypes PPSSSS and PPSSPS with haplotypes
PSS and PSP of PrP gene at the codon numbers 42, 138, and 240. All four breeds showed both wild type monomorphic sequence
and mutant polymorphic sequences of these codons. The mutants of 42 and 138 codons translate the same amino acids as with
the wild type sequences, while the mutant of codon 240 is responsible for a different amino acid translation i.e., serine
to proline. In short, this study provides preliminary information about alleles and genotypes of PrP gene in four goat breeds
of Pakistan. |
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Keywords: | Scrapie PrP Goat Pakistan |
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