Cold stress induced high molecular weight membrane polypeptides are responsible for cold tolerance in Rhizobium DDSS69 |
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Authors: | Nagesh Sardesai Cherukeri Raghavendra Babu |
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Affiliation: | Department of Botany, University of Delhi, India. nageshsardesai@hotmail.com |
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Abstract: | Cold stress induces a lag phase in the growth cycle of Rhizobium DDSS69. Two cold sensitive mutants of DDSS69 were generated through Tn5 tagged mutagenesis. These mutants do not grow below 15 degrees C but show a growth curve comparable with the wild type grown at 5 degrees C. There is a rapid induction of two high molecular weight membrane polypeptides of 135 and 119 kDa within 15 min of exposure to 5 degrees C in DDSS69. PAGE membrane protein profiles of stressed and non-stressed cells reveal differential regulation of genes. At 15 degrees C both mutants lack the high molecular weight polypeptides, suggesting a role in alleviation of cold stress. |
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Keywords: | cold stress Rhizobium cold induced polypeptides |
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