A biochemical characterization of feline MHC products: Unusually high expression of class 11 antigens on peripheral blood lymphocytes |
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| Authors: | Jacques J. Neefjes Evert J. Hensen Toon I. P. de Kroon Hidde L. Ploegh |
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| Affiliation: | (1) The Netherlands Cancer Institute, Amsterdam, The Netherlands;(2) Department of Immunology, Faculty of Veterinary Medicine, University of Utrecht, Utrecht, The Netherlands |
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| Abstract: | The polymorphism of feline MHC antigens was examined using biochemical methods. The following observations were made: (1) feline class I and II antigens are polymorphic. Their biochemical features were established using rabbit and mouse reagents directed against human MHC products; they resemble those observed for other mammalian species; (2) the expression of class II antigens in unstimulated cat peripheral blood lymphocytes (PBLs) appears to be unusually high. Cat PBLs express far more class II than class I antigens, whereas in human Epstein-Barr virus-transformed lines, which are known to express relatively large amounts of class II antigens, the situation is reversed.Abbreviations used in this paper EBV Epstein-Barr virus - FLA feline lymphocyte antigen - MHC major histocompatibility complex - MLC mixed lymphocyte culture - MLR mixed lymphocyte reaction - PBL peripheral blood lymphocyte - RT room temperature - TX-114 Triton X-114 - 1-D IEF one-dimensional isoclectric focusing - 2-D SDS-PAGE twodimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
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