The Rice OsRad21-4, an Orthologue of Yeast Rec8 Protein,is Required for Efficient Meiosis |
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Authors: | Liangran?Zhang Jiayi?Tao Shunxin?Wang Kang?Chong Email author" target="_blank">Tai?WangEmail author |
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Institution: | (1) Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China |
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Abstract: | In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister
chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our
identification revealed that the rice genome has 4 RAD21-like genes that share lower than 21% identity at polypeptide levels, and each is present as a single copy in this genome.
Here we describe our analysis of the function of OsRAD21-4 by RNAi. Western blot analyses indicated that the protein was most abundant in young flowers and less in leaves and buds
but absent in roots. In flowers, the expression was further defined to premeiotic pollen mother cells (PMCs) and meiotic PMCs
of anthers. Meiotic chromosome behaviors were monitored from male meiocytes of OsRAD21-4-deficient lines mediated by RNAi.
The male meiocytes showed multiple aberrant events at meiotic prophase I, including over-condensation of chromosomes, precocious
segregation of homologues and chromosome fragmentation. Fluorescence in situ hybridization experiments revealed that the deficient lines were defective in homologous pairing and cohesion at sister chromatid
arms. These defects resulted in unequal chromosome segregation and aberrant spore generation. These observations suggest that
OsRad21-4 is essential for efficient meiosis. |
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Keywords: | cohesion condensation meiosis OsRAD21-4 Oryza sativa pairing |
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