| Abstract: | Genetically transformed roots and calli were induced from leafsegments of grapevine (Vitis vinifera L. cv. Koshusanjaku) afterco-cultivation with wild-type Agrobacterium rhizogenes strains,but plant regenera tion from them was not achieved. On the otherhand, transgenlc grapevine plants were obtained via somaticembryogenesis after co-cultivation of embryogenic calli withan engineered A. rhizogenes strain including both the neomycinphosphotransferase II (NPT II) and the ß-glucuronidase(GUS) genes, followed by selection of secondary embryos forkanamycin resistance. All these plants showed GUS gene expressionrevealed by histochemical assay. Southern blot analysis revealedthe stable integration of the GUS cording region in their genome.Transformants containing Ri T-DNA exhibited various phenotypes:most of them showed a typical Ri-transformed phenotype suchas wrinkled leaves, while the others looked normal. Key words: Agrobacterium rhizogenes, grapevine, transgenic plants, Vitis vinifera |
