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Detection of Cochliobolus heterostrophus races in South China
Authors:Meng Wang  Shaoqing Wang  Jia Ma  Chuanjin Yu  Jinxin Gao  Jie Chen
Institution:1. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China;2. State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University/Laboratory of Southern Urban Agriculture of Ministry of Agriculture, Shanghai, China;3. Institute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, Tangshan, China
Abstract:Cochliobolus heterostrophus is the causal pathogen of the southern corn leaf blight (SCLB). There are three known races: race O, race C and race T. To determine which Cheterostrophus races comprise the field population in southern China and to assess diversity of these strains in terms of virulence, 200 isolates from diseased plants were collected in nine provinces/municipalities. All were race O, that is, no race T or race C isolates were found. Sixty race O isolates that sporulated well were chosen for further analysis. Virulence was measured using the integral optical density (IOD) of leaf lesions on four maize inbred lines. UPGMA cluster analysis of AFLP markers was applied to the 60 race O isolates plus control race O, T and C strains. Phylogenetic distribution, geographic location and virulence were not correlated. These results can provide valuable information for guidance in early warning and disease control.
Keywords:AFLP     Cochliobolus heterostrophus     IOD  races
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