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Control of Pratylenchus brachyurus in soybean with Trichoderma spp. and resistance inducers
Authors:Juliana Kath  Cláudia R. Dias‐Arieira  Júlio César Antunes Ferreira  Juliana Aparecida Homiak  Camila Rocco da Silva  Carine Rezende Cardoso
Affiliation:1. Post Graduate in Agronomy, State University of Maringa, Maringa, PR, Brazil;2. Department of Agronomic Science, State University of Maringá, Umuarama, PR, Brazil;3. Department of Biologial Control, Grupo Farroupilha, Patos de Minas, MG, Brazil
Abstract:Trichoderma spp. is a fungus with nematode control potential; however, its potential to control the root lesion nematode Pratylenchus brachyurus remains poorly studied. Thus, the aim of this study was to select Trichoderma spp. isolates and assess their ability to control P. brachyurus in soybean crops. Different experiments were conducted aiming at selecting isolates, assessing whether they were able to reduce nematode penetration in plants or cause mortality in vitro, and whether they were able to induce resistance in soybean, as well as at studying the possibility of using the selected isolates associated with resistance inducers (acibenzolar‐S‐methyl, Ecolife? and AgroMos?). The selection experiment found three isolates showing satisfactory results, namely GF422, GF425 and GF427; the GF362 isolate was assessed in the subsequent experiments. These four isolates reduced P. brachyurus penetration in soybean roots and promoted nematode mortality in vitro. Increased total protein and catalase activity were recorded, mainly in the 72‐hr assessments. Overall, the protein production was different between isolates. The best results were found in the combination between the GF362 isolate and the three resistance inducers, between GF427 and Ecolife?, between GF427 and AgroMos? and between GF422 and Ecolife?.
Keywords:biological control     Glycine max     nematophagous fungi  resistance induction  root lesion nematode
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