Circular dichroism study of 2'-5' dinucleoside monophosphates modified with N-2-acetylaminofluorene. |
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Authors: | M Boublik D Grunberger Y Lapidot |
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Institution: | 1. Roche Institute of Molecular Biology, Nutley, N.J. 07110 U.S.A.;2. Intitute of Cancer Research and Department of Biochemistry, Columbia University College of Physicians and Surgeons, N.Y. U.S.A.;3. Department of Biological Chemistry, The Hebrew University of Jerusalem, Israel |
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Abstract: | The conformational properties of four 2′ – 5′ dinucleoside monophosphates modified with -2-acetylaminofluorene have been studied by circular dichroism spectroscopy. Covalent binding of this chemical carcinogen at the C8 position of guanosine in the 2′ – 5′ dinucleoside monophosphates induces striking changes in their circular dichroic spectra depending on their base sequence and composition. The changes in CD spectra, redshift of the extrema and change of their polarity, not observed in the spectra of corresponding 3′ – 5′ derivatives modified with -2-acetylaminofluorene are correlated with the difference in the configuration of 2′ – 5′ and 3′ – 5′ dinucleoside monophosphates and discussed in respect to the intramolecular stacking interactions. |
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Keywords: | guanosine modified with N-2-acetylaminofluorene AcNH-Fln N-2-acetylaminofluorene AcN(OAc)-Fln N-acetoxy-2-acetylaminofluorene |
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