Micropropagation of pitaya (<Emphasis Type="Italic">Hylocereus undatus</Emphasis> Britton et rose) |
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Authors: | Email author" target="_blank">Yasseen?Mohamed-YasseenEmail author |
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Institution: | (1) Genetic Engineering and Biotechnology Research Institute, Menoufiya University, P.O. Box 79/22857, Sadat City, Egypt |
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Abstract: | Summary A procedure for micropropagation of pitaya using thidiazuron (TDZ) and naphthaleneacetic acid (NAA) is described. Explants
were excised from young joints of mature plants and cultured on Murashige and Skoog medium (MS) containing 0.5 μM NAA and 0.5 μM TDZ. Shoots produced from these first explants were cut up to produce secondary explants, either by decapitation or by longitudinal
division into three parts. The decapitated and longitudinal explants were cultured on MS supplemented with 0.5 μM NAA and either 0.01, 0.09, 0.5, or 0.9 μM TDZ. Decapitated explants produced more shoots at higher frequency that the longitudinal explants. For both types of secondary
explants, most shoots were developed from the distal parts. Shoots produced from secondary explants were rooted in MS and
then transferred to soil where they produced normal plants. |
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Keywords: | cactus explant nigh-blooming pitahaya propagation strawberry pear thidiazuron tropical fruits |
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