Cloning of the replication region on the bacteriocinogenic plasmid pRJ9 of Staphylococcus aureus |
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| Authors: | Selma Soares de Oliveira Sandra Amaral de Araújo Maria do Carmo de Freire Bastos |
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| Institution: | Departamento de Microbiologia Geral, Instituto de Microbiologia da UFRJ, CCS, Cidade Universitára, Rio de Janero, Brazil |
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| Abstract: | Abstract A 5.8-kb Cla I fragment of pRJ9, a bacteriocinogenic plasmid of Sphylococcus aureus , was cloned in the unique Cla I site of pRJ5. The recombinant plasmid obtained, pRJ23, failed to confer bacteriocin production and immunity to bacteriocin on host cells. The cloned fragment was shown to contain the complete replicon of pRJ9. Attempts to clone the 4.4-kb Cla I fragment of pRJ9 were unsuccessful, apparently due to the inactivation of the basic replicon of the cloning vector. Therefore, plasmid pRJ5 cut at its Cla I site appears to be a suitable vector for cloning replication regions of plasmids that cab replicate in S. aureus . |
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| Keywords: | Staphylococcus aureus Bacteriocinogenic plasmid Gene cloning Replication region |
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