| 应用FP-TDI技术进行高通量单核苷酸多态分型 |
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| 引用本文： | 罗春清,邓立彬,周珺,林伟,曾长青. 应用FP-TDI技术进行高通量单核苷酸多态分型[J]. 生物化学与生物物理进展, 2004, 31(8): 731-735 |
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| 作者姓名： | 罗春清 邓立彬 周珺 林伟 曾长青 |
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| 作者单位： | 1. 中国科学院基因组学研究所暨北京华大基因研究中心,北京,101300;中国科学院遗传与发育生物学研究所,北京,100101
2. 中国科学院基因组学研究所暨北京华大基因研究中心,北京,101300 |
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| 基金项目： | “十五”国家重大科技专项（2002BA711A09）和中国科学院知识创新工程重大项目(KSCX2-SW-207). |
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| 摘 要： | FP-TDI (fluorescence polarization template-directed dye-terminator incorporation）是一种操作简单、实验投入少、适于高通量反应的单核苷酸多态等位基因分型技术.使用两种评价分型图像质量的数值指标，可以有效地对分型结果进行评价，使该技术得到了改进.在此基础上优化了实验条件，并应用该技术，对人类基因组3号染色体上随机选取的337个单核苷酸多态性位点进行了高通量分型，反应的一次成功率达到59.94%.
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| 关 键 词： | 单核苷酸多态 FP-TDI 基因分型 |
| 收稿时间： | 2004-02-17 |
| 修稿时间： | 2004-03-28 |
High Throughput SNP Genotyping With FP-TDI |
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| LUO Chun-Qing,DENG Li-Bin,ZHOU Jun,LIN Wei and ZENG Chang-Qing. High Throughput SNP Genotyping With FP-TDI[J]. Progress In Biochemistry and Biophysics, 2004, 31(8): 731-735 |
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| Authors: | LUO Chun-Qing DENG Li-Bin ZHOU Jun LIN Wei ZENG Chang-Qing |
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| Affiliation: | Beijing Genomics Institute, The Chinese Academy of Sciences, Beijing 101300, China;The Institute of Genetic and Developmental Biology, The Chinese Academy of Sciences, Beijing 100101, China;Beijing Genomics Institute, The Chinese Academy of Sciences, Beijing 101300, China;Beijing Genomics Institute, The Chinese Academy of Sciences, Beijing 101300, China;Beijing Genomics Institute, The Chinese Academy of Sciences, Beijing 101300, China;Beijing Genomics Institute, The Chinese Academy of Sciences, Beijing 101300, China |
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| Abstract: | Fluorescence polarization template-directed dye-terminator incorporation(FP-TDI) is a simple, cheap and high throughput SNP (single nucleotide polymorphism) genotyping technology, but the quality of genotyping pictures cannot be evaluated, thus the reaction condition is very difficult to optimize. A numeric standard which can efficiently evaluate the SNP scoring picture was introduced, based on this standard, the reaction system was optimized. Using this method, randomly selected 337 SNP locus of human chromosome 3 were applied to high throughput SNP genotyping, the first time success rate reached 59.94%. |
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| Keywords: | single nucleotide polymorphism(SNP) FP-TDI genotyping optimization |
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