Changes in Isozyme Patterns During in Vitro Regeneration from Cotyledon Explants of Brassica Species |
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Authors: | S Samantaray GR Rout P Das |
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Institution: | (1) Plant Physiology and Biochemistry Laboratory, Regional Plant Resource Centre, Bhubaneswar, 751 015 Orissa, India;(2) Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar -, 751 015 Orissa, India |
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Abstract: | High frequency shoot regeneration from cotyledons excised from 4-d-old seedlings of Brassica campestris L. cv. M 27 and Brassica
juncea (L.) Czern. cv. Pusabold was achieved on Murashige and Skoog's (MS) medium supplemented with 1.0 mg dm-3 N6-benzyladenine (BA) and 3 % (m/v) saccharose. Rooting occurred simultaneously with shoot formation on the medium containing
1.0 mg dm-3 BA and 0.5 mg dm-3 1-naphthaleneacetic acid. Cultures of cotyledon, cotyledon derived non-differentiating calli and differentiated calli with
shoots and/or roots were analysed at different intervals for isozyme patterns of esterase and peroxidase. With the BA-induced
development of shoot and/or root from non-differentiating callus, some conspicuous isozymes appeared which indicates the involvement
of these isozymes in root and shoot development rather than in induction of morphogenesis in callus.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | esterase growth regulators PAGE peroxidase |
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