| 重组铜绿假单胞菌外毒素A工程菌的高密度发酵与表达 |
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| 引用本文： | 董勇前,宣俊文,冯宜扬,丁浩,范锋锋,胡海涛,赵志强.重组铜绿假单胞菌外毒素A工程菌的高密度发酵与表达[J].微生物学免疫学进展,2011,39(1):24-27. |
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| 作者姓名： | 董勇前 宣俊文 冯宜扬 丁浩 范锋锋 胡海涛 赵志强 |
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| 作者单位： | 兰州生物制品研究所,兰州,730046 |
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| 摘 要： | 高密度培养表达大肠杆菌生产重组铜绿假单胞菌外毒素A(rEPA)。用上海高机公司的30L自控发酵罐,采用分批补料培养技术,维持葡萄糖的浓度始终处于较低的水平,并分批补加氮源,同时溶氧控制在30%～40%,pH自动调控至7.0,培养至对数中期进行诱导。重组菌最终发酵液光密度(A600)未诱导时达到44,诱导时达到36,在上清液中表达的rEPA蛋白的含量占总蛋白的28.1%,在菌体中表达蛋白含量占总蛋白的4.7%。本实验为rE-PA的大规模生产奠定了基础。
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| 关 键 词： | 铜绿假单胞菌外毒素A 高密度培养 表达 |
High cell density culture of recombinant E.coli to produce Pseudomonas aeruginosa exoprotein A |
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| DONG Yong-qian,XUAN Jun-wen,FENG Yi-yang,et al..High cell density culture of recombinant E.coli to produce Pseudomonas aeruginosa exoprotein A[J].Progress In Microbiology and Immunology,2011,39(1):24-27. |
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| Authors: | DONG Yong-qian XUAN Jun-wen FENG Yi-yang |
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| Institution: | DONG Yong-qian,XUAN Jun-wen,FENG Yi-yang,et al.(Lanzhou Institute of Biological Products,Lznzhou 730046,China) |
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| Abstract: | To culture recombinant E.coli in high cell density to produce Pseudomonas aeruginosa exoprotein A(rEPA).30L autocontrol fermenter made by Shanghai GaoJi Corperation and fed-batch culture technology were used in this experiment,adding nitrogen nutrition in batches,keeping glucose concentration at low level,dissolved oxygen at 30% to 40%,pH at about 7.00 throughout the culture and induced at middle stage of logarithm growth stage.When not induced,the final cell density is 44,when induced,the final cell densit... |
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| Keywords: | Pseudomonas aeruginosa exoprotein A High cell density culture Expression |
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