Chlorate inhibits tyrosine sulfation of human type III procollagen without affecting its secretion or processing |
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Authors: | A Jukkola J Risteli L Risteli |
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Affiliation: | Department of Medical Biochemistry, University of Oulu, Finland. |
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Abstract: | Sodium chlorate, a potent inhibitor of sulfation reactions, completely inhibits the formation of tyrosine-o-sulfate in type III procollagen in human fibroblasts, when used in concentrations that do not affect the incorporation of radioactive amino acids into protein. The unsulfated type III procollagen is secreted into the medium at a rate comparable, to those of sulfated type III procollagen and type I procollagen, which normally does not undergo sulfation. The enzymatic cleavage of the aminoterminal propeptide of type III procollagen is incomplete in fibroblast cultures, irrespective of the sulfation status of the protein. |
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