Fractionation of synaptophysin-containing vesicles from rat brain and cultured PC12 pheochromocytoma cells |
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Authors: | Bertram Wiedenmann Hubert Rehm Martin Knierim Cord-Michael Becker |
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Institution: | Department of Internal Medicine, Ludolf-Krehl-Klinik, University of Heidelberg, FRG. |
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Abstract: | Synaptophysin is a transmembrane glycoprotein of neuroendocrine vesicles. Its content and distribution in subcellular fractions from cultured PC12 cells, rat brain and bovine adrenal medulla were determined by a sensitive dot immunoassay. Synaptophysin-containing fractions appeared as monodispersed populations similar to synaptic vesicles in density and size distribution. Membranes from synaptic vesicles contained approximately 100-times more synaptophysin than chromaffin granules. In conclusion, synaptophysin is located almost exclusively in vesicles of brain and PC12 cells which are distinct from dense core granules. |
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Keywords: | Synaptophysin Synaptic vesicle Dense core vesicle Dot immunoassay |
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