Search for A-factor-Dependent Variants in Actinomycete Populations |
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Authors: | O V Efremenkova V D Gruzina I G Sumarukova V D Kuznetsov |
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Institution: | (1) Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, ul. Bol'shaya Pirogovskaya 11, Moscow, 119021, Russia;(2) Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia |
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Abstract: | A study of 28 nocardia-like, asporogenous, and oligosporous spontaneous morphological variants belonging to 23 species of streptomycetes revealed five strains producing regulators of the A-factor group. Streptomyces griseus 1439, which forms aerial mycelium and spores only in the presence of exogenous A-factor, was used as the test strain. Among the 28 spontaneous variants, three new A-factor-dependent strains were revealed, which represented the species Streptomyces griseus, S. citreofluorescens, and S. viridovulgaris subsp. albomarinus. These weakly differentiated variants did not produce A-factor and behaved as its recipients, responding by changes in their morphological characteristics at a concentration of this regulator in the medium of 0.01 g/ml or higher. The original collection strains in whose populations the variants were selected produced substances of the A-factor group. The A-factor-dependent variants differed in the level of the regulator required for maximal expression of the morphological characteristics: it was necessary to introduce the A-factor at a concentration of 1 g/ml for S. citreofluorescens and S. viridovulgaris subsp. albomarinus and at 10 g/ml for S. griseus. |
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Keywords: | A-factor regulators of the A-factor group actinomycetes streptomycetes cytodifferentiation A-factor-dependent variants |
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