Rapid screening of peptides for heavy metal binding |
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Authors: | Kotrba Pavel Dolečková Lucie Pavlík Manfred Ruml Tomáš |
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Institution: | (1) Dept. Biochem. and Microbiol., Faculty of Food and Biochem. Technology, ICT Prague, Technická 3, 166 28 Prague, Czech Republic;(2) Dept. Biochem., Inst. Org. Chem. and Biochem. Academy of Sciences, Flemingovo n. 2, 166 10 Prague, Czech Republic |
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Abstract: | Summary A simple method that allows testing and characterisation of cadmium binding motifs by large set of immobilized peptides was developed. Hepta- and octapeptides containing cysteine and histidine residues were synthesized on cotton carrier. They were tested for cadmium binding. pH values of half metal dissociation were measured. Test for Cd binding was based on its precipitation with ferrous dipyridyl iodide. Subsequent dissolving of precipitate provided semiquantitative data on relative amount of Cd bound. Observed data for particular peptides corresponded with prediction.Abbreviations
Boc
tercial butyloxycarboyl
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Fmoc
fluorenylmethyloxycarboyl
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tBu
tercial butyl
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DMF
N,N-dimethylformamide
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DCM
dichlormethane
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Me
methyl |
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Keywords: | |
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