粘虫飞行过程中四种相关酶的活性变化

对3日龄粘虫雌蛾吊飞过程中4种相关酶3-羟酰辅酶A脱氢酶(HOAD)、3-磷酸甘油醛脱氢酶(GAPDH)、3-磷酸甘油脱氢酶(GDH)和乳酸脱氢酶(LDH)的研究结果表明，在室内条件下，粘虫在吊飞过程中其能量代谢有以下特点： 在吊飞的初始5 min，所有与糖代谢和脂肪代谢相关的酶活性都快速升高，这段时期脂肪代谢的酶活性也完全被活化，HOAD活性明显增强；但在随后的5～60 min持续吊飞期间与能量代谢有关的酶活性都有所下降，表明此时飞行活性趋于平稳。飞行中的粘虫具有极高的有氧代谢能力，也具备一定的无氧代谢能力。吊飞过程中HOAD∶GAPDH大于1，说明粘虫飞行过程中能源物质利用属于混合型，但动用脂肪比糖类要多。

Changes in activities of four energy metabolism related enzymes during flight of Mythimna separata

 The effect of the flight on the activities of energy metabolism related enzymes in the flight muscles of the Oriental armyworm moth, Mythimna separata females was studied. These enzymes measured were glyceraldehydes phosphate dehydrogenase (GAPDH), glycerol-3-phosphate dehydrogenase (GDH), lactate dehydrogenase (LDH), and 3-hydroxyacyl-CoA dehydrogenase (HOAD). In the first 5 min of the tethered-flight of 3 day-old moth, activities of all the enzymes related to carbohydrate and lipid metabolism increased rapidly,enzymes related to lipid metabolism were completely activated, and the activities of HOAD were significantly strengthened. But in flight duration from 5th to 60th min, the activities of all energymetabolism related enzymes decreased,suggesting that the flight was going to be stabilized. High levels of GDH and LDH activities during flight suggested that both aerobic and anaerobic metabolism happened in flight muscle. The ratio of HOAD∶GAPDH suggested that not only lipids but also carbohydrates in the female could be involved as energy substrates in the process of flight activity.