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Identification and characterization of yeast isolated from indonesian fermented food
Authors:Hiroshi Kuriyama  Dudi Sastraatmadja  Yoko Igosaki  Kaoru Watanabe  Atit Kanti  Takema Fukatsu
Institution:(1) National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, 1-1, Higashi, 305 Tsukuba, Ibaraki, Japan;(2) Research and Development Centre for Biology, The Indonesian Institute of Science, J1.Ir. Juanda 18, 16002 Bogor, Indonesia
Abstract:Yeast strains with amylolytic activity were isolated from cassavatapé and its precursor,ragi. they were divided into two groups based on their characteristics: group 1, possessing high amylolytic activity and low ethanol yield; and group 2, possessing low amylolytic activity and high ethanol yield. The major strains of the group 1 were identified asEndomyces fibuliger, and those of group 2 were identified asPichia anomala. Based on 18S rDNA analysis, an isolate fromragi that had a high amylolytic activity was thought to be an undescribed species that was related to the basidiomycetous genera.
Keywords:18S rDNA analysis  Indonesian fermented food            ragi                      tapé            yeast identification
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