| 也门铁的组织培养技术研究 |
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| 引用本文： | 齐安民,王家琼,李楠,贺涛,李薇,崔大方. 也门铁的组织培养技术研究[J]. 亚热带植物科学, 2015, 44(1): 23-28. DOI: 10.3969/j.issn.1009-7791.2015.01.005 |
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| 作者姓名： | 齐安民 王家琼 李楠 贺涛 李薇 崔大方 |
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| 作者单位： | （1.华南农业大学 林学院，广东 广州 510642；2.重庆云阳县果品产业发展局，重庆 云阳 404500；3.重庆奉节县林业局，重庆 奉节 404600；4.华南农业大学 公共基础课实验教学中心，广东 广州510642；5.广州南沙明珠湾区开发有限公司，广东 广州 511455） |
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| 摘 要： | 以4品种也门铁的茎段为外植体进行组织培养技术研究。结果表明，控制普通也门铁茎段褐化效果最理想的培养基为MS + 0.25 g·L-1 VC + 0.50 g·L-1 Na2S2O3；诱导也门铁不定芽萌动的最佳培养基为MS + 3.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.1 mg·L-1 KT；诱导也门铁不定芽增殖的最佳培养基，因品种不同而异，普通也门铁和金心也门铁为MS + 2.0 mg·L-1 6-BA + 1.0 mg·L-1 NAA + 0.1 mg·L-1 GA3，扭纹铁和金心扭纹铁为MS + 1.0 mg·L-1 6-BA + 0.5 mg·L-1 NAA + 0.1 mg·L-1 GA3；也门铁壮苗的最佳培养基为MS + 0.05 mg·L-1 6-BA + 0.05 mg·L-1 NAA + l g·L-1AC；也门铁生根最优培养基为1/2MS + 0.5 mg·L-1 IBA。
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| 关 键 词： | 也门铁 组织培养 褐化 |
| 收稿时间： | 2014-11-26 |
| 修稿时间： | 2015-04-18 |
Tissue Culture Technique of Dracaena fragrans |
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| QI An-min;WANG Jia-qiong;LI Nan;HE Tao;LI Wei;CUI Da-fang. Tissue Culture Technique of Dracaena fragrans[J]. Subtropical Plant Science, 2015, 44(1): 23-28. DOI: 10.3969/j.issn.1009-7791.2015.01.005 |
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| Authors: | QI An-min WANG Jia-qiong LI Nan HE Tao LI Wei CUI Da-fang |
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| Affiliation: | (1.College of Forestry, South China Agricultural University, Guangzhou 510642, Guangdong China; 2.Yunyang Fruit Industry Development Council, Yunyang 404500, Chongqing China; 3.Fengjie Forestry Bureau, Fengjie 404600, Chongqing China; 4. Center of Experimental Teaching for common Basic Courses, South China Agricultural University, Guangzhou 510642, Guangdong China; 5.Nansha Pearl Bay Development Co. Ltd., Guangzhou 511455, Guangdong China ) |
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| Abstract: | The tissue culture techniques of four cultivars of Dracaena fragrans used stem section as the explant were analyzed. A series of important conclusions can be made: 1) The optimal medium for controlling tissue browning of D. fragrans: MS + 0.25 g·L-1 VC + 0.50 g·L-1 Na2S2O3; 2) The optimal medium for inducing the D. fragrans stem stirring: MS+ 3.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.1 mg·L-1 KT; 3) The optimal proliferation medium of D. fragrans, depends on the cultivars of D. fragrans. The optimal medium for D. fragrans and D. fragrans ‘Massangeana’: MS + 2.0 m g·L-1 6-BA + 1.0 mg·L-1 NAA + 0.1 mg·L-1 GA3; The optimal medium for D. fragrans ‘Compacta’ and D. fragrans ‘Janet-craig’: MS + 1.0 m g·L-1 6-BA + 0.5 mg·L-1 NAA + 0.1 mg·L-1 GA3; 4) Under the condition of MS + 0.05 mg·L-1 6-BA + 0.05 mg·L-1 NAA + l g·L-1 AC was the most efficient the Dracaena fragrans seedling medium; 5) 1/2MS + 0.5 mg·L-1 IBA was the most efficient rooting medium. |
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| Keywords: | Dracaena fragrans tissue culture callus browning |
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