Eotaxin induces migration of RBL-2H3 mast cells via a Rac-ERK-dependent pathway |
| |
Authors: | Woo Chang-Hoon Jeong Dong Tak Yoon Seog-Beom Kim Key-Sun Chung Il Yup Saeki Toshihiko Kim Jae-Hong |
| |
Affiliation: | Graduate School of Biotechnology, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul 136-701, South Korea. |
| |
Abstract: | Eotaxin is a potent chemokine that acts via CC chemokine receptor 3 (CCR3) to induce chemotaxis, mainly on eosinophils. Here we show that eotaxin also induces chemotactic migration in rat basophilic leukemia (RBL-2H3) mast cells. This effect was dose-dependently inhibited by compound X, a selective CCR3 antagonist, indicating that, as in eosinophils, the effect was mediated by CCR3. Eotaxin-induced cell migration was completely blocked in RBL-RacN17 cells expressing a dominant negative Rac1 mutant, suggesting a crucial role for Rac1 in eotaxin signaling to chemotactic migration. ERK activation also proved essential for eotaxin signaling and it too was absent in RBL-RacN17 cells. Finally, we found that activation of Rac and ERK was correlated with eotaxin-induced actin reorganization known to be necessary for cell motility. It thus appears that Rac1 acts upstream of ERK to signal chemotaxis in these cells, and that a Rac-ERK-dependent cascade mediates the eotaxin-induced chemotactic motility of RBL-2H3 mast cells. |
| |
Keywords: | Eotaxin Chemotaxis RBL-2H3 Rac ERK |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|