Reconstitution of 50 S ribosomal subunits from Bacillus stearothermophilus with 5 S RNA from spinach chloroplasts and low-Mr RNA from mitochondria of Locusta migratoria and bovine liver |
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| Authors: | D W Vogel R K Hartmann M Bartsch A R Subramanian W Kleinow T W O'Brien T Pieler V A Erdmann |
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| Affiliation: | 1. Institut für Biochemie, Freie Universität Berlin, Thielallee 69-73, D-1000 Berlin 33, Germany;2. Max-Planck-Institut für Molekulare Genetik, Ihnestraße 63-73, D-1000 Berlin 33, Germany;3. II. Lehrstuhl: Tierphysiologie, Weyertal 119, D-5000 Köln 41, FRG;4. Department of Biochemistry and Molecular Biology, Box J-245, University of Florida, Gainesville, FL 32610, USA |
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| Abstract: | Reconstitution experiments with 50 S ribosomal subunits from Bacillus stearothermophilus demonstrate that spinach chloroplast 5 S rRNA can be incorporated into the bacterial ribosome and yield biologically active particles, thereby establishing the eubacterial nature of chloroplast 5 S rRNA. In contrast, mitochondria from Locusta migratoria or bovine liver do not appear to contain discrete, low-Mr RNAs, which can replace 5 S rRNA in the functional reconstitution of B. stearothermophilus ribosomes. |
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| Keywords: | Chloroplast 5 S rRNA Mitochondrial 5 S rRNA 50 S ribosome Reconstitution Protein synthesis Evolution |
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