| 沙打旺原生质体培养再生植株 |
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| 引用本文： | 罗希明 何孟元. 沙打旺原生质体培养再生植株[J]. 遗传学报, 1991, 18(3): 239-243 |
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| 作者姓名： | 罗希明 何孟元 |
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| 作者单位： | 吉林省农业科学院大豆研究所，吉林省农业科学院大豆研究所，吉林省农业科学院大豆研究所，吉林省农业科学院大豆研究所，东北师范大学生物系，东北师范大学生物系 公主岭 136100 东北师范大学生物系，公主岭 136100，公主岭 136100，公主岭 136100，长春，长春 |
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| 摘 要： | 用1%半纤维素酶,0.4%纤维素酶,0.1%果胶离析酶,CPW9M酶液分离沙打旺无菌苗下胚轴和子叶原生质体。K8P原生质体培养基悬滴培养。下胚轴原生质体形成小细胞团后用琼脂糖包埋培养,形成小块愈伤组织后转入增殖培养基M1、M2(改良MS培养基)上形成大块愈伤组织。经过两次诱导分化,在分化培养基M3(MS 0.7mg/L BA 0.2mg/L NAA),M4(MS 0.5mg/L BA 0.5mg/L KT 0.5mg/L ZT 0.2mg/L NAA)和M6(MS 3mg/L ZT 0.2mg/L IAA)上分化出苗,再生植株。由子叶分离的原生质体未能形成愈伤组织。
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| 关 键 词： | 沙打旺 原生质体培养 再生植株 |
Plant Regeneration from Protoplast Culture of Astragalus huangheensis |
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| Luo Ximing Zhao Guelan Xie Xueju Liu Yianzhi. Plant Regeneration from Protoplast Culture of Astragalus huangheensis[J]. Journal of Genetics and Genomics, 1991, 18(3): 239-243 |
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| Authors: | Luo Ximing Zhao Guelan Xie Xueju Liu Yianzhi |
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| Abstract: | Protoplasts were enzymatically isolated from hypocotyls of Astragalus huangheensis. The protoplasts formed calli in K8P liquid medium. The calli were transferred onto Ml medium (MS l-2mg/L 2,4-D 0.5mg/L ZT 0.2mg/L NAA) and M2 medium (MS 0.5-1mg/L 2.4-D 0.7mg/L BA 0.2mg/L NAA) to form big calli. Shoot formation was initiated on M3 medium (MS 0.7mg/L BA 0.2mg/L NAA), M4 medium (MS 0.5mg/L ZT 0.5mg/L BA 0.5mg/L KT 0.2mg/L NAA) and M6 medium (MS 3mg/L ZT 0.2mg/L NAA). Plants were regenerated from protoplasts of hypocotyls. |
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| Keywords: | Astragalus huanghecnsis Protoplast culture Plant regeneration |
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