Evaluation of time-resolved fluoroimmunoassay with Eu-labelled protein-A for serum progesterone |
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| Authors: | A Ius L Ferrara G Meroni M A Bacigalupo |
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| Affiliation: | Istituto di Chimica degli Ormoni, C.N.R., Milano, Italy. |
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| Abstract: | Progesterone has been assayed in several serum samples by a time-resolved fluoroimmunoassay (TR-FIA). The solid phase was 11 alpha-hydroxyprogesterone hemisuccinate bound covalently to ovalbumin and adsorbed on wells of polystyrene. The assay was based on competitive reaction of solid phase-bound hormone and samples with specific antibody labelled in situ with protein-A prelabelled with europium. The bound Eu was dissociated from the solid phase by an enhancement solution and measured by time-resolved fluorometry. The coefficient of correlation between TR-FIA and RIA was 0.97. |
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