Production of human immunodeficiency virus from T-lymphoblastoid cells using serum-free medium |
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Authors: | Bok-Hwan Chun Ilye-Sook Kim Eui-Cheol Jo Song-Yong Park Dong-Il Kim Sung Bok Paik |
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Institution: | (1) MOGAM Biotechnology Research Institute, 341 Pojung-Ri, Koosung-Myon, 449-910 Yongin-Kun, Kyonggi-Do, Korea |
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Abstract: | Summary A serum-free medium was constructed for the suspension culture of mammalian cells. Effects of the serum-free medium on the cultivation of T-lymphoblastoid cells secreting human immunodeficiency virus (HIV) were observed. The cell growth and HIV production in the serum-free medium were comparable to those in the medium supplemented with 10% fetal bovine serum (FBS), which showed a possibility of economical industrial application. |
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