Lack of excision of 4HAQO adducts from DNA by cell extracts that excise pyrimidine dimers |
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| Authors: | G B Panigrahi I G Walker |
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| Affiliation: | 1. Waters Corporation, Milford, 01757 MA, USA;2. MTM Research Centre, Örebro University, SE-701 82, Örebro, Sweden;3. Atlantic Ecology Division, U.S. Environmental Protection Agency, Narragansett, RI 02882, USA;4. Wellington Laboratories Inc., Guelph, N1G 3M5 ON, Canada;5. Scientialis Consulting LLC, Hopkinton, 01748 MA, USA;1. Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China;2. School of Pharmacy, China Medical University, Shenyang, 110122, China;1. Department of Dermatology, Copenhagen University Hospital – Bispebjerg and Frederiksberg, Nielsine Nielsens Vej 17, 2400 Copenhagen, Denmark;2. Department of Pharmacy, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark;1. LEO Pharma A/S, Ballerup, Denmark;2. Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark;1. Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2100 Copenhagen, Denmark;2. Department of Health Technology, Technical University of Denmark, DK-2800 Lyngby, Denmark;4. Bioneer:FARMA, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2100 Copenhagen, Denmark |
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| Abstract: | A substrate of DNA containing 4HAQO adducts, suitable for studies of excision repair, was prepared by reacting calf thymus DNA with [3H]monoacetyl-4HAQO. A crude HeLa cell extract was prepared by the method of Mortelmans et al (Proc. Natl. Acad. Sci. U.S.A. 73, 2757, 1976). The cell extract would specifically excise pyrimidine dimers from UV-irradiated DNA but would not release 4HAQO adducts in an acid soluble form. This result points to different initial steps in the excision repair process for these two forms of damage even though much of the repair mechanism is common to both. |
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