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Spotting effect in microarray experiments |
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| Institution: | 1.Institut National Agronomique Paris-Grignon,France;2.UMR Génomique Végétale,INRA-CNRS-Université d'Evry,F-91057 Evry,France;3.Laboratoire d'Immunologie Virale,Institut Pasteur,Paris,France;4.Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB),Ghent University,Gent,Belgium |
| Abstract: | BackgroundMicroarray data must be normalized because they suffer from multiple biases. We have identified a source of spatial experimental variability that significantly affects data obtained with Cy3/Cy5 spotted glass arrays. It yields a periodic pattern altering both signal (Cy3/Cy5 ratio) and intensity across the array. |
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