Subcellular compartmentation of proline in the leaves of the subantarctic Kerguelen cabbage Pringlea antiscorbutica R. Br. In vivo<sup><nobr>13</nobr></sup>C-NMR study期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Subcellular compartmentation of proline in the leaves of the subantarctic Kerguelen cabbage Pringlea antiscorbutica R. Br. In vivo¹³C-NMR study

Authors:	S AUBERT F HENNION A BOUCHEREAU E GOUT R BLIGNY & A-J DORNE

Institution:	Laboratoire de Physiologie Cellulaire Végétale, U.R.A. 576 (CNRS) and,;Laboratoire de Résonance Magnétique en Biologie Métabolique, DBMS, Commissariat àl'Energie Atomique (CEA-Grenoble), UniversitéJoseph Fourier, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France, and,;Groupe de Biologie Végétale, UMR 6553 Ecobio (CNRS), Universitéde Rennes I, 35042 Rennes Cedex, France

Abstract:	Proline is one of the major solutes accumulated upon salt stress in leaves, stem and roots of the subantarctic Brassicaceae Pringlea antiscorbutica R. Br. (Kerguelen cabbage). Using in vivo ¹³C-NMR techniques, it was possible for the first time to visualize the subcellular compartmentation of proline between cytoplasmic and vacuolar compartments in Pringlea leaves. We observed that this osmolyte accumulated at a 2–3 times higher concentration in the cytoplasm than in the vacuole.

Keywords:	Pringlea antiscorbutica in vivo ¹³C-NMR Kerguelen archipelago proline salt stress subcellular compartmentation