A fluorescent histochemical method for the detection of tissue disulphide groups |
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| Authors: | T. Adesanya Ige Grillo Dorothea L. Baxter-Grillo |
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| Affiliation: | (1) Department of Anatomy, University of Ibadan, Ibadan, Nigeria |
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| Abstract: | Summary Bennett and Yphantis (1948) introduced into histochemistry the use of organic mercurials for the detection of sulphydryl groups. The new fluorescent method which is now reported is similarly based upon the reaction of mercaptans with an organic mercurial, fluorescein mercuric acetate (FMA). This method is sensitive; moreover it is not as elaborate as the dihydroxy-dinaphthyl-disulphide (DDD) technique of Barrnett and Seligman (1952). |
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